Genomic and genetic industries are considered among the fastest growing biotech markets in the world, yet the underlying DNA analysis results are often inaccurate or misleading due to samples with damaged DNA and artifacts from storage.

Problem:
Difficult FFPE Samples

While FFPE samples can be stored stably at room temperature for years, the fixation process creates inter- and intra-strand cross-links in the DNA. These cross-links prevent amplification of the sample and can render certain parts of the genome inaccessible or even entire failure of the sequencing run.

Solution: BioCule

Problem:
poor RNA-Seq Results

 Transcriptomics applications, like RNA-Seq and RNA microarrays, require a series of enzymatic steps resulting in the creation of cDNA libraries. Libraries contaminated with DNA/RNA hybrids and ssDNA will throw off conventional QC methods, leading to an overestimation of library material.

Solution: BioCule

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Problem:
Library Cloning Failure

The enzymes involved in cloning DNA molecules require dsDNA. Samples contaminated with ssDNA can result in either lower yields or even complete failure of the library.




Solution: BioCule

BUT HOW DO YOU KNOW IF YOUR SAMPLES HAVE DNA DAMAGE AND WHAT KIND OF DAMAGE IT IS?

BioCule offers you a way to accurately assess your DNA quality

 

Having an accurate assessment of the level and type of DNA damage that your samples may or may not have can save a lot of time, failure, cost and effort.

With the BioCule technology you can quickly assess the level of e.g cross-links, ssDNA, fragmentation, DNA/RNA hybrids, and strand breaks.

  This insight to the quality of your DNA samples gives you the information you need to:

  • Adjust your processes to account for e.g. cross-linked DNA or DNA damage
  • Save time and money by discarding ruined samples rather than analyzing them
  • Improve the quality of your results by only working with high quality samples